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29 mer scrambled shrna cassette  (OriGene)


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    Structured Review

    OriGene 29 mer scrambled shrna cassette
    29 Mer Scrambled Shrna Cassette, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mer+scrambled+shrna+cassette/TMEM85+(EMC4)+Human+shRNA+Plasmid+Kit/pm41911287-123-13-21
    Average 94 stars, based on 2 article reviews
    29 mer scrambled shrna cassette - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    shRNA:

    Article Title: Intracellular delivery of antiviral shRNA using penetratin-based complexes effectively inhibits respiratory syncytial virus replication and host cell apoptosis.
    Article Snippet: The validated recombinant plasmids were amplified in E. coli DH5-α cells and purified using the GF1 nucleic acid extraction kit (Vivantis Technologies Co. Malaysia) according to the manufacturer’s instructions. .. A noneffective 29-mer scrambled shRNA cassette in pGFPV-RS vector (CAT#: TR30013, OriGene Technologies, USA) served as a negative control. .. Preparation of penetratin/pGFP-V-RS containing shRNA complexes Penetratin (RQIKIWFQNRRMKWKK) was synthesized by Proteogenix (France) with over 95% purity.

    Article Title: An epigenetic switch controls an alternative NR2F2 isoform that unleashes a metastatic program in melanoma
    Article Snippet: NR2F2-isoform 1 knockdown was performed by short hairpin interference using two shRNAs: shNR2F2-Iso1-X and shNR2F2-Iso1-Y (HSH018031, GeneCopeia). .. Non-targeting 29-mer Scrambled shRNA Cassette in pGFP-C-shLenti Vector (TR30021, Origene) was used as control. .. Lentiviral vectors were also used for NR2F2 ectopic expression: NR2F2 Isoform 1 (EX-C0221-Lv205, GeneCopoeia) and NR2F2 Isoform 2 (RC226609L2, Origene). pEZ-Lv205 (EX-NEG-Lv205, GeneCopoeia) or pLenti-C-mGFP (PS100071, Origene) were used as control vectors.

    Article Title: Identification of regulatory elements in primary sensory neurons involved in neuropathic pain
    Article Snippet: Stable knockdown of Cebpg in 50B11 cells was done by transfecting 50B11 cells with pGFP-C-shLenti carrying shRNA against Cebpg (1μg/ml; TL709448; Origene, Rockville, MD) following manufacturer’s recommendations. .. A 29-mer scrambled shRNA cassette in the pGFP-C-shLenti vector (TR30021; Origene) was used as the off-target control. ..

    Article Title: Platelet-derived growth factor-α receptor is the cellular receptor for human cytomegalovirus gHgLgO trimer
    Article Snippet: .. Down regulations of ErbB1, ErbB2 and PDGFRα were done by lentiviral transduction of cells using 4 unique 29mer shRNA constructs in pGFP-C-shLenti and 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector (Origene) using manufacturer’s instructions. ..

    Article Title: Nrf2 deficiency exacerbates ochratoxin A-induced toxicity in vitro and in vivo.
    Article Snippet: Several mechanisms are postulated to be responsible for nephrotoxic and nephrocarcinogenic activities of mycotoxin and food contaminant, ochratoxin A (OTA).. Although Nrf2 transcription factor was suggested to be involved in OTA–mediated renal injury, comprehensive study evaluating the effect of OTA toxicity in Nrf2 knock-out mice with special regard to sex-dependency has not been performed yet.. Our results clearly show exacerbated OTA toxicity in porcine tubular epithelial cells after shRNA-mediated Nrf2 inhibition as well as in proximal tubular cells isolated from Nrf2male mice in comparison to cells derived from their wild-type counterparts.

    Article Title: An epigenetic switch controls an alternative NR2F2 isoform that unleashes a metastatic program in melanoma.
    Article Snippet: NR2F2-isoform 1 knockdown was performed by short hairpin interference using two shRNAs: shNR2F2-Iso1-X and shNR2F2-Iso1-Y (HSH018031, GeneCopeia). .. Nontargeting 29-mer Scrambled shRNA Cassette in pGFP-C-shLenti Vector (TR30021, Origene) was used as control. .. Lentiviral vectors were also used for NR2F2 ectopic expression: NR2F2 Isoform 1 (EX-C0221-Lv205, GeneCopoeia) and NR2F2 Isoform 2 (RC226609L2, Origene). pEZLv205 (EX-NEG-Lv205, GeneCopoeia) or pLenti-C-mGFP (PS100071, Origene) were used as control vectors.

    Article Title: Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development
    Article Snippet: .. As control for α2δ-knockdown experiments, a noneffective 29-mer scrambled shRNA cassette cloned into pGFP-C-shLenti vector (catalog #TR30021; OriGene Technologies) was used. ..

    Plasmid Preparation:

    Article Title: Intracellular delivery of antiviral shRNA using penetratin-based complexes effectively inhibits respiratory syncytial virus replication and host cell apoptosis.
    Article Snippet: The validated recombinant plasmids were amplified in E. coli DH5-α cells and purified using the GF1 nucleic acid extraction kit (Vivantis Technologies Co. Malaysia) according to the manufacturer’s instructions. .. A noneffective 29-mer scrambled shRNA cassette in pGFPV-RS vector (CAT#: TR30013, OriGene Technologies, USA) served as a negative control. .. Preparation of penetratin/pGFP-V-RS containing shRNA complexes Penetratin (RQIKIWFQNRRMKWKK) was synthesized by Proteogenix (France) with over 95% purity.

    Article Title: Identification of regulatory elements in primary sensory neurons involved in neuropathic pain
    Article Snippet: Stable knockdown of Cebpg in 50B11 cells was done by transfecting 50B11 cells with pGFP-C-shLenti carrying shRNA against Cebpg (1μg/ml; TL709448; Origene, Rockville, MD) following manufacturer’s recommendations. .. A 29-mer scrambled shRNA cassette in the pGFP-C-shLenti vector (TR30021; Origene) was used as the off-target control. ..

    Article Title: Nrf2 deficiency exacerbates ochratoxin A-induced toxicity in vitro and in vivo.
    Article Snippet: Several mechanisms are postulated to be responsible for nephrotoxic and nephrocarcinogenic activities of mycotoxin and food contaminant, ochratoxin A (OTA).. Although Nrf2 transcription factor was suggested to be involved in OTA–mediated renal injury, comprehensive study evaluating the effect of OTA toxicity in Nrf2 knock-out mice with special regard to sex-dependency has not been performed yet.. Our results clearly show exacerbated OTA toxicity in porcine tubular epithelial cells after shRNA-mediated Nrf2 inhibition as well as in proximal tubular cells isolated from Nrf2male mice in comparison to cells derived from their wild-type counterparts.

    Negative Control:

    Article Title: Intracellular delivery of antiviral shRNA using penetratin-based complexes effectively inhibits respiratory syncytial virus replication and host cell apoptosis.
    Article Snippet: The validated recombinant plasmids were amplified in E. coli DH5-α cells and purified using the GF1 nucleic acid extraction kit (Vivantis Technologies Co. Malaysia) according to the manufacturer’s instructions. .. A noneffective 29-mer scrambled shRNA cassette in pGFPV-RS vector (CAT#: TR30013, OriGene Technologies, USA) served as a negative control. .. Preparation of penetratin/pGFP-V-RS containing shRNA complexes Penetratin (RQIKIWFQNRRMKWKK) was synthesized by Proteogenix (France) with over 95% purity.

    Control:

    Article Title: An epigenetic switch controls an alternative NR2F2 isoform that unleashes a metastatic program in melanoma
    Article Snippet: NR2F2-isoform 1 knockdown was performed by short hairpin interference using two shRNAs: shNR2F2-Iso1-X and shNR2F2-Iso1-Y (HSH018031, GeneCopeia). .. Non-targeting 29-mer Scrambled shRNA Cassette in pGFP-C-shLenti Vector (TR30021, Origene) was used as control. .. Lentiviral vectors were also used for NR2F2 ectopic expression: NR2F2 Isoform 1 (EX-C0221-Lv205, GeneCopoeia) and NR2F2 Isoform 2 (RC226609L2, Origene). pEZ-Lv205 (EX-NEG-Lv205, GeneCopoeia) or pLenti-C-mGFP (PS100071, Origene) were used as control vectors.

    Article Title: Identification of regulatory elements in primary sensory neurons involved in neuropathic pain
    Article Snippet: Stable knockdown of Cebpg in 50B11 cells was done by transfecting 50B11 cells with pGFP-C-shLenti carrying shRNA against Cebpg (1μg/ml; TL709448; Origene, Rockville, MD) following manufacturer’s recommendations. .. A 29-mer scrambled shRNA cassette in the pGFP-C-shLenti vector (TR30021; Origene) was used as the off-target control. ..

    Article Title: An epigenetic switch controls an alternative NR2F2 isoform that unleashes a metastatic program in melanoma.
    Article Snippet: NR2F2-isoform 1 knockdown was performed by short hairpin interference using two shRNAs: shNR2F2-Iso1-X and shNR2F2-Iso1-Y (HSH018031, GeneCopeia). .. Nontargeting 29-mer Scrambled shRNA Cassette in pGFP-C-shLenti Vector (TR30021, Origene) was used as control. .. Lentiviral vectors were also used for NR2F2 ectopic expression: NR2F2 Isoform 1 (EX-C0221-Lv205, GeneCopoeia) and NR2F2 Isoform 2 (RC226609L2, Origene). pEZLv205 (EX-NEG-Lv205, GeneCopoeia) or pLenti-C-mGFP (PS100071, Origene) were used as control vectors.

    Article Title: Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development
    Article Snippet: .. As control for α2δ-knockdown experiments, a noneffective 29-mer scrambled shRNA cassette cloned into pGFP-C-shLenti vector (catalog #TR30021; OriGene Technologies) was used. ..

    Transduction:

    Article Title: Platelet-derived growth factor-α receptor is the cellular receptor for human cytomegalovirus gHgLgO trimer
    Article Snippet: .. Down regulations of ErbB1, ErbB2 and PDGFRα were done by lentiviral transduction of cells using 4 unique 29mer shRNA constructs in pGFP-C-shLenti and 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector (Origene) using manufacturer’s instructions. ..

    Article Title: Nrf2 deficiency exacerbates ochratoxin A-induced toxicity in vitro and in vivo.
    Article Snippet: Several mechanisms are postulated to be responsible for nephrotoxic and nephrocarcinogenic activities of mycotoxin and food contaminant, ochratoxin A (OTA).. Although Nrf2 transcription factor was suggested to be involved in OTA–mediated renal injury, comprehensive study evaluating the effect of OTA toxicity in Nrf2 knock-out mice with special regard to sex-dependency has not been performed yet.. Our results clearly show exacerbated OTA toxicity in porcine tubular epithelial cells after shRNA-mediated Nrf2 inhibition as well as in proximal tubular cells isolated from Nrf2male mice in comparison to cells derived from their wild-type counterparts.

    Construct:

    Article Title: Platelet-derived growth factor-α receptor is the cellular receptor for human cytomegalovirus gHgLgO trimer
    Article Snippet: .. Down regulations of ErbB1, ErbB2 and PDGFRα were done by lentiviral transduction of cells using 4 unique 29mer shRNA constructs in pGFP-C-shLenti and 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector (Origene) using manufacturer’s instructions. ..

    Article Title: Nrf2 deficiency exacerbates ochratoxin A-induced toxicity in vitro and in vivo.
    Article Snippet: Several mechanisms are postulated to be responsible for nephrotoxic and nephrocarcinogenic activities of mycotoxin and food contaminant, ochratoxin A (OTA).. Although Nrf2 transcription factor was suggested to be involved in OTA–mediated renal injury, comprehensive study evaluating the effect of OTA toxicity in Nrf2 knock-out mice with special regard to sex-dependency has not been performed yet.. Our results clearly show exacerbated OTA toxicity in porcine tubular epithelial cells after shRNA-mediated Nrf2 inhibition as well as in proximal tubular cells isolated from Nrf2male mice in comparison to cells derived from their wild-type counterparts.

    Clone Assay:

    Article Title: Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development
    Article Snippet: .. As control for α2δ-knockdown experiments, a noneffective 29-mer scrambled shRNA cassette cloned into pGFP-C-shLenti vector (catalog #TR30021; OriGene Technologies) was used. ..



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